Please use this identifier to cite or link to this item:
|Title:||Diffusion controlled and temperature stable microcapsule reaction compartments for high-throughput microcapsule-PCR|
|Authors:||Mak, W.C. |
|Source:||Mak, W.C.,Cheung, K.Y.,Trau, D. (2008-10-09). Diffusion controlled and temperature stable microcapsule reaction compartments for high-throughput microcapsule-PCR. Advanced Functional Materials 18 (19) : 2930-2937. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200800388|
|Abstract:||A novel approach to perform a high number of individual polymerase chain reactions (PCR) in microcapsule reaction compartments, termed "Microcapsule-PCR" was developed. Temperature stable microcapsules with a selective permeable capsule wall were constructed by matrix-assisted layer-by-layer (LbL) Encapsulation technique. During the PCR, small molecular weight building blocks - nucleotides (dNTPs) were supplied externally and diffuse through the permeable capsule wall into the interior, while the resulted high molecular weight PCR products were accumulated within the microcapsule. Microcapsules (∼110.8 μm average diameter) filled with a PCR reaction mixture were constructed by an emulsion technique having a 2% agarose core and a capsule formed by LbL coating with poly(allylanune-hydrochloride) and poly(4-styrene-sulfonate). An encapsulation efficiency of 47% (measured for primer-FITC (22 bases)) and 98% PCR efficiency was achieved. Microcapsules formed by eight layers of polyelectrolyte and subjected to PCR cycling (up to 95°C) demonstrated good temperature stability without any significantly changes in DNA retention yield and microcapsule morphology. A multiplex Microcapsule-PCR experiment demonstrated that microcapsules are individual compartment and do not exchange templates or primers between microcapsules during PCR cycling. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA.|
|Source Title:||Advanced Functional Materials|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 8, 2018
checked on Jan 20, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.