Please use this identifier to cite or link to this item:
|Title:||Composite electrospun scaffolds for engineering tubular bone grafts|
|Authors:||Ekaputra, A.K. |
|Citation:||Ekaputra, A.K., Zhou, Y., Cool, S.M., Hutmacher, D.W. (2009-12-01). Composite electrospun scaffolds for engineering tubular bone grafts. Tissue Engineering - Part A 15 (12) : 3779-3788. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2009.0186|
|Abstract:||In this study, poly (ε-caprolactone) [PCL] and its collagen composite blend (PCL/Col) were fabricated to scaffolds using electrospinning method. Incorporated collagen was present on the surface of the fibers, and it modulated the attachment and proliferation of pig bone marrow mesenchymal cells (pBMMCs). Osteogenic differentiation markers were more pronounced when these cells were cultured on PCL/Col fibrous meshes, as determined by immunohistochemistry for collagen type I, osteopontin, and osteocalcin. Matrix mineralization was observed only on osteogenically induced PCL/Col constructs. Long bone analogs were created by wrapping osteogenic cell sheets around the PCL/Col meshes to form hollow cylindrical cell-scaffold constructs. Culturing these constructs under dynamic conditions enhanced bone-like tissue formation and mechanical strength. We conclude that electrospun PCL/Col mesh is a promising material for bone engineering applications. Its combination with osteogenic cell sheets offers a novel and promising strategy for engineering of tubular bone analogs. © 2009, Mary Ann Liebert, Inc.|
|Source Title:||Tissue Engineering - Part A|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 16, 2018
WEB OF SCIENCETM
checked on Jul 31, 2018
checked on Aug 17, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.