Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tea.2009.0186
Title: Composite electrospun scaffolds for engineering tubular bone grafts
Authors: Ekaputra, A.K. 
Zhou, Y.
Cool, S.M.
Hutmacher, D.W.
Issue Date: 1-Dec-2009
Citation: Ekaputra, A.K., Zhou, Y., Cool, S.M., Hutmacher, D.W. (2009-12-01). Composite electrospun scaffolds for engineering tubular bone grafts. Tissue Engineering - Part A 15 (12) : 3779-3788. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2009.0186
Abstract: In this study, poly (ε-caprolactone) [PCL] and its collagen composite blend (PCL/Col) were fabricated to scaffolds using electrospinning method. Incorporated collagen was present on the surface of the fibers, and it modulated the attachment and proliferation of pig bone marrow mesenchymal cells (pBMMCs). Osteogenic differentiation markers were more pronounced when these cells were cultured on PCL/Col fibrous meshes, as determined by immunohistochemistry for collagen type I, osteopontin, and osteocalcin. Matrix mineralization was observed only on osteogenically induced PCL/Col constructs. Long bone analogs were created by wrapping osteogenic cell sheets around the PCL/Col meshes to form hollow cylindrical cell-scaffold constructs. Culturing these constructs under dynamic conditions enhanced bone-like tissue formation and mechanical strength. We conclude that electrospun PCL/Col mesh is a promising material for bone engineering applications. Its combination with osteogenic cell sheets offers a novel and promising strategy for engineering of tubular bone analogs. © 2009, Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/66976
ISSN: 19373341
DOI: 10.1089/ten.tea.2009.0186
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

53
checked on May 22, 2018

WEB OF SCIENCETM
Citations

48
checked on May 7, 2018

Page view(s)

27
checked on May 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.