Please use this identifier to cite or link to this item: https://doi.org/10.1002/stem.221
Title: Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells
Authors: Ng, V.Y.
Ang, S.N.
Chan, J.X.
Choo, A.B.H. 
Keywords: Cell adhesion molecules
Cell surface markers
Embryonic stem cells
Monoclonal antibodies
Issue Date: Jan-2010
Source: Ng, V.Y., Ang, S.N., Chan, J.X., Choo, A.B.H. (2010-01). Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells. Stem Cells 28 (1) : 29-35. ScholarBank@NUS Repository. https://doi.org/10.1002/stem.221
Abstract: Human embryonic stem cells (hESCs) have the capacity to remain pluripotent and self-renew indefinitely. To discover novel players in the maintenance of hESCs, we have previously reported the generation of monoclonal antibodies that bind to cell surface markers on hESCs, and not to mouse embryonic stem cells or differentiated embryoid bodies. In this study, we have identified the antigen target of one such monoclonal antibody as the epithelial cell adhesion molecule (EpCAM). In undifferentiated hESCs, EpCAM is localized to Octamer 4 (OCT4)-positive pluripotent cells, and its expression is down-regulated upon differentiation. To further understand its biological function in hESCs, endogenous EpCAM expression was silenced using small interfering RNA. EpCAM knockdown had marginal negative effects on OCT4 and TRA-1-60 expression, however cell proliferation was decreased by >40%. Examination of lineage marker expression showed marked upregulation of endoderm and mesoderm genes in EpCAM-silenced cells, under both pluripotent and differentiating conditions. These results were validated using a hESC line whose EpCAM expression has been stably knocked down. Data from the stable line confirmed that downregulation of EpCAM decreases cell growth and increases gene expression in the endoderm and mesoderm lineages. In vivo, hESCs lacking EpCAM were able to form teratomas containing tissues representing the three germ layers, and gene expression analysis yielded marked increase in the endoderm marker alpha fetoprotein compared with control. Together these data demonstrate that EpCAM is a surface marker on undifferentiated hESCs and plays functional roles in proliferation and differentiation. © AlphaMed Press.
Source Title: Stem Cells
URI: http://scholarbank.nus.edu.sg/handle/10635/66959
ISSN: 10665099
DOI: 10.1002/stem.221
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

48
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

40
checked on Nov 21, 2017

Page view(s)

38
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.