Please use this identifier to cite or link to this item:
|Title:||Assembly of polystyrene microspheres and its application in cell micropatterning|
|Citation:||Yap, F.L., Zhang, Y. (2007-05). Assembly of polystyrene microspheres and its application in cell micropatterning. Biomaterials 28 (14) : 2328-2338. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.01.034|
|Abstract:||Cell micropatterning has important applications in the development of biosensors and lab-on-a-chip devices, tissue engineering and fundamental cell biology studies. The conventional micropatterning techniques involve patterning of cells over a planar substrate. In this paper, we propose the introduction of topographical features on cell adhesive regions to enhance cell adhesion and function. The textured surface is created by assembly of polystyrene microspheres and the topographical parameters can be varied systematically by changing the size and density of the particles. A technique of generating spatial arrangement of microspheres on a nonfouling background is developed. This is achieved by using a bi-functional template which has a patterned hydrophobic parylene film to facilitate self-assembly of particles; after assembly, the film is liftoff, revealing a cell resistance background which is compatible with cell micropatterning. Particles were assembled by selective wetting of the hydrophobic-hydrophilic template. A fluidic chamber was designed to control the movement of the particle suspension across the template so as to attain uniform particle array over large area. This method of cell micropatterning can improve the efficiency and functionality of cell-based devices. It can also be used for examining the effects of topographical cues on cell-substrate adhesion which can provide valuable insights into cell biology and design of biomaterials. © 2007 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2018
WEB OF SCIENCETM
checked on Jun 20, 2018
checked on May 12, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.