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Title: Study on the enzymatic hydrolysis of racemic methyl ibuprofen ester
Authors: Venu Madhav, M.
Ching, C.B. 
Keywords: Candida rugosa
Fed batch reactor
Issue Date: 2001
Source: Venu Madhav, M., Ching, C.B. (2001). Study on the enzymatic hydrolysis of racemic methyl ibuprofen ester. Journal of Chemical Technology and Biotechnology 76 (9) : 941-948. ScholarBank@NUS Repository.
Abstract: The hydrolysis of racemic methyl ibuprofen ester in the presence of lipase from Candida rugosa was investigated in shake flasks. Experiments were performed to study the effect of temperature, pH and shaking speed on the reaction rate. Different hydrophobic co-solvents were screened for the highest reaction rate and the presence of enzyme inhibition by substrate and products was examined. A kinetic expression was then proposed to describe the reaction. Kinetic parameters were determined for the optimum operating conditions and the proposed model was verified with the experimental results. Next, this reaction was scaled up to a fed batch stirred tank reactor. Batch reactor and fed batch reactor configurations were compared for better conversions. The effects of aqueous phase hold-up, substrate concentration and feed flow rate on the conversion of the reaction were also studied. Higher conversions were obtained in a fed batch reactor when compared with the batch reactor. In the fed batch reactor, increased conversions were observed with lower feed flowrates and high aqueous phase hold-up. © 2001 Society of Chemical Industry.
Source Title: Journal of Chemical Technology and Biotechnology
ISSN: 02682575
DOI: 10.1002/jctb.466
Appears in Collections:Staff Publications

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