Please use this identifier to cite or link to this item: https://doi.org/10.1007/s003740000332
Title: Measurement of dehydrogenase activity using 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) in the presence of copper
Authors: Obbard, J.P. 
Keywords: 2,3,5-Triphenyltetrazolium chloride
2-p-Iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride
2-p-Iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium formazan
Copper-contaminated soil
Microbial dehydrogenase activity
Issue Date: 2001
Source: Obbard, J.P. (2001). Measurement of dehydrogenase activity using 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) in the presence of copper. Biology and Fertility of Soils 33 (4) : 328-330. ScholarBank@NUS Repository. https://doi.org/10.1007/s003740000332
Abstract: This paper reports on the adverse effect of Cu on the use of 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) as a substrate for measuring microbial dehydrogenase activity (DHA). The presence of Cu affected the absorbance of the reaction product 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium form The experiment provides evidence that the use of INT is an unreliable measure of DHA activity in soils contaminated with Cu.
Source Title: Biology and Fertility of Soils
URI: http://scholarbank.nus.edu.sg/handle/10635/66656
ISSN: 01782762
DOI: 10.1007/s003740000332
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