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|Title:||Measurement of dehydrogenase activity using 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) in the presence of copper|
Microbial dehydrogenase activity
|Citation:||Obbard, J.P. (2001). Measurement of dehydrogenase activity using 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) in the presence of copper. Biology and Fertility of Soils 33 (4) : 328-330. ScholarBank@NUS Repository. https://doi.org/10.1007/s003740000332|
|Abstract:||This paper reports on the adverse effect of Cu on the use of 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) as a substrate for measuring microbial dehydrogenase activity (DHA). The presence of Cu affected the absorbance of the reaction product 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium form The experiment provides evidence that the use of INT is an unreliable measure of DHA activity in soils contaminated with Cu.|
|Source Title:||Biology and Fertility of Soils|
|Appears in Collections:||Staff Publications|
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