Determination of the adsorption isotherm of fluoxetine by adjustable factor h-root method (AF-HRM)

Abstract

Conventional methods to determine competitive adsorption isotherm are tedious, and require large quantities of pure isomers. In some cases pure enantiomers are not available, thereby making the full experiments to determine the competitive adsorption isotherm of enantiomers impossible. A new method, adjustable factor h-root method (AF-HRM), had been presented for the measurement of Langmuir equilibrium coefficients. In the new approach, the retention factors and the frontal retention factors are required to determine the parameters of isotherms. As the ultimate goal is to separate enantiomers of fluoxetine on a preparative scale, a convenient method to determine the adsorption isotherm is essential. In this paper, we have demonstrated that AF-HRM can be applied to determine the parameters in the extended Langmuir model. Experimental results agree well with the prediction.