Please use this identifier to cite or link to this item: https://doi.org/10.2175/106143008X276651
Title: Alternative Immunofluorescent labeling of cryptosporidium parvum in water samples using semiconductor quantum dots
Authors: Lee, L.Y. 
Hu, J.Y. 
Ong, S.L. 
Ng, H.Y. 
Wong, S.W.
Feng, Y. 
Tan, X.
Keywords: Cryptosporidium parvum
Immunofluorescent labels
Oocysts
Organic fluorophores
Quantum dot
Issue Date: Aug-2008
Source: Lee, L.Y., Hu, J.Y., Ong, S.L., Ng, H.Y., Wong, S.W., Feng, Y., Tan, X. (2008-08). Alternative Immunofluorescent labeling of cryptosporidium parvum in water samples using semiconductor quantum dots. Water Environment Research 80 (8) : 725-731. ScholarBank@NUS Repository. https://doi.org/10.2175/106143008X276651
Abstract: The application of immunofluorescent labeling using quantum dots for detection of inactivated Cryptosporidium parvum oocysts in spiked water samples (reservoir water, treated wastewater effluent, permeate of a membrane bioreactor, and tap water) provided more consistent results compared with the organic fluorophores label. The varying degree of particles present in the different water samples (with turbidity ranging from 0.2 to 6.1 NTU) in nonconcentrated water samples had insignificant interference on the labeled counts (2-sample t-tests, p < 0.236) using the quantum dot label, while the quantum dot label provided an advantage of approximately 50% lower interference in concentrated water samples compared with the organic fluorophores label.
Source Title: Water Environment Research
URI: http://scholarbank.nus.edu.sg/handle/10635/65108
ISSN: 10614303
DOI: 10.2175/106143008X276651
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