Please use this identifier to cite or link to this item:
|Title:||Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease|
|Authors:||Li, A. |
Free fatty acid
|Source:||Li, A., Ngo, T.P.N., Yan, J., Tian, K., Li, Z. (2012-06). Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresource Technology 114 : 725-729. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.03.034|
|Abstract:||A whole-cell based solvent-free system was developed for efficient conversion of waste grease to biodiesel via one-pot esterification and transesterification. By isolation and screening of lipase-producing strains from soil, Serratia marcescens YXJ-1002 was discovered for the biotransformation of grease to biodiesel. The lipase (SML) from this strain was cloned and expressed in Escherichia coli as an intracellular enzyme, showing 6 times higher whole-cell based hydrolysis activity than that of wild type strain. The recombinant cells were used for biodiesel production from waste grease in one-pot reactions containing no solvent with the addition of methanol in several small portions, and 97% yield of biodiesel (FAME) was achieved under optimized conditions. In addition, the whole-cell biocatalysts showed excellent reusability, retaining 74% productivity after 4 cycles. The developed system, biocatalyst, and process enable the efficient, low-cost, and green production of biodiesel from waste grease, providing with a potential industrial application. © 2012 Elsevier Ltd.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 7, 2018
WEB OF SCIENCETM
checked on Jan 24, 2018
checked on Mar 11, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.