Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.03.034
Title: Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease
Authors: Li, A. 
Ngo, T.P.N.
Yan, J.
Tian, K.
Li, Z. 
Keywords: Biodiesel
Biotransformation
Free fatty acid
Grease
Whole-cell
Issue Date: Jun-2012
Source: Li, A., Ngo, T.P.N., Yan, J., Tian, K., Li, Z. (2012-06). Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresource Technology 114 : 725-729. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.03.034
Abstract: A whole-cell based solvent-free system was developed for efficient conversion of waste grease to biodiesel via one-pot esterification and transesterification. By isolation and screening of lipase-producing strains from soil, Serratia marcescens YXJ-1002 was discovered for the biotransformation of grease to biodiesel. The lipase (SML) from this strain was cloned and expressed in Escherichia coli as an intracellular enzyme, showing 6 times higher whole-cell based hydrolysis activity than that of wild type strain. The recombinant cells were used for biodiesel production from waste grease in one-pot reactions containing no solvent with the addition of methanol in several small portions, and 97% yield of biodiesel (FAME) was achieved under optimized conditions. In addition, the whole-cell biocatalysts showed excellent reusability, retaining 74% productivity after 4 cycles. The developed system, biocatalyst, and process enable the efficient, low-cost, and green production of biodiesel from waste grease, providing with a potential industrial application. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/64796
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.03.034
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