Please use this identifier to cite or link to this item:
https://doi.org/10.1002/asia.200900297
DC Field | Value | |
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dc.title | Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A | |
dc.contributor.author | Shi, J. | |
dc.contributor.author | Cai, L. | |
dc.contributor.author | Pu, K.Y. | |
dc.contributor.author | Liu, B. | |
dc.date.accessioned | 2014-06-17T07:49:51Z | |
dc.date.available | 2014-06-17T07:49:51Z | |
dc.date.issued | 2010-02-01 | |
dc.identifier.citation | Shi, J., Cai, L., Pu, K.Y., Liu, B. (2010-02-01). Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A. Chemistry - An Asian Journal 5 (2) : 301-308. ScholarBank@NUS Repository. https://doi.org/10.1002/asia.200900297 | |
dc.identifier.issn | 18614728 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/64663 | |
dc.description.abstract | A neutral polyfluorene derivative that contains 20 mol% 2,1,3-benzothiadiazole (BT) is synthesized by Suzuki cross-coupling polymerization. A cationic conjugated polymer A and an a-mannose-bearing polymer B are subsequently obtained through different post-polymerization methods. As a result of the charged pendant groups or sugar-bearing groups attached to the polymer side chains, both A and B show good water-solubility. The titration of Concanavalin A (Con A) into polymer aqueous solution leads to different fluorescent responses for polymers A and B. Polymer A does not show any obvious fluorescence change upon interaction with Con A, whereas polymer B shows fluorescence increase in BT emission intensity when Con a is added. This is because of the specific interaction between a-mannose and Con A, which induces polymer aggregation, and then facilitates energy transfer from the phenylene-fluorine segments to the BT units. A practical calibration curve ranging from 1 nm to 250 nm is obtained by correlating the changes in BT emission intensity with Con A concentration. The advantage of polymer B-based Con A macromolecular probe is that it shows signal increase upon Con A recognition, which is significantly different from other conjugated polymer-based fluorescence quenching assays. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/asia.200900297 | |
dc.source | Scopus | |
dc.subject | Benzothiadiazole | |
dc.subject | Biosensors | |
dc.subject | Click chemistry | |
dc.subject | FRET | |
dc.subject | Polymers | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/asia.200900297 | |
dc.description.sourcetitle | Chemistry - An Asian Journal | |
dc.description.volume | 5 | |
dc.description.issue | 2 | |
dc.description.page | 301-308 | |
dc.identifier.isiut | 000274539100013 | |
Appears in Collections: | Staff Publications |
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