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Title: Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A
Authors: Shi, J. 
Cai, L. 
Pu, K.Y. 
Liu, B. 
Keywords: Benzothiadiazole
Click chemistry
Issue Date: 1-Feb-2010
Citation: Shi, J., Cai, L., Pu, K.Y., Liu, B. (2010-02-01). Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A. Chemistry - An Asian Journal 5 (2) : 301-308. ScholarBank@NUS Repository.
Abstract: A neutral polyfluorene derivative that contains 20 mol% 2,1,3-benzothiadiazole (BT) is synthesized by Suzuki cross-coupling polymerization. A cationic conjugated polymer A and an a-mannose-bearing polymer B are subsequently obtained through different post-polymerization methods. As a result of the charged pendant groups or sugar-bearing groups attached to the polymer side chains, both A and B show good water-solubility. The titration of Concanavalin A (Con A) into polymer aqueous solution leads to different fluorescent responses for polymers A and B. Polymer A does not show any obvious fluorescence change upon interaction with Con A, whereas polymer B shows fluorescence increase in BT emission intensity when Con a is added. This is because of the specific interaction between a-mannose and Con A, which induces polymer aggregation, and then facilitates energy transfer from the phenylene-fluorine segments to the BT units. A practical calibration curve ranging from 1 nm to 250 nm is obtained by correlating the changes in BT emission intensity with Con A concentration. The advantage of polymer B-based Con A macromolecular probe is that it shows signal increase upon Con A recognition, which is significantly different from other conjugated polymer-based fluorescence quenching assays. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
Source Title: Chemistry - An Asian Journal
ISSN: 18614728
DOI: 10.1002/asia.200900297
Appears in Collections:Staff Publications

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