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|Title:||Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A|
|Authors:||Shi, J. |
|Citation:||Shi, J., Cai, L., Pu, K.Y., Liu, B. (2010-02-01). Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A. Chemistry - An Asian Journal 5 (2) : 301-308. ScholarBank@NUS Repository. https://doi.org/10.1002/asia.200900297|
|Abstract:||A neutral polyfluorene derivative that contains 20 mol% 2,1,3-benzothiadiazole (BT) is synthesized by Suzuki cross-coupling polymerization. A cationic conjugated polymer A and an a-mannose-bearing polymer B are subsequently obtained through different post-polymerization methods. As a result of the charged pendant groups or sugar-bearing groups attached to the polymer side chains, both A and B show good water-solubility. The titration of Concanavalin A (Con A) into polymer aqueous solution leads to different fluorescent responses for polymers A and B. Polymer A does not show any obvious fluorescence change upon interaction with Con A, whereas polymer B shows fluorescence increase in BT emission intensity when Con a is added. This is because of the specific interaction between a-mannose and Con A, which induces polymer aggregation, and then facilitates energy transfer from the phenylene-fluorine segments to the BT units. A practical calibration curve ranging from 1 nm to 250 nm is obtained by correlating the changes in BT emission intensity with Con A concentration. The advantage of polymer B-based Con A macromolecular probe is that it shows signal increase upon Con A recognition, which is significantly different from other conjugated polymer-based fluorescence quenching assays. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.|
|Source Title:||Chemistry - An Asian Journal|
|Appears in Collections:||Staff Publications|
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