Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/64490
Title: Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics
Authors: Shi, H.
Kwok, R.T.K.
Liu, J.
Xing, B.
Tang, B.Z.
Liu, B. 
Issue Date: 31-Oct-2012
Citation: Shi, H., Kwok, R.T.K., Liu, J., Xing, B., Tang, B.Z., Liu, B. (2012-10-31). Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics. Journal of the American Chemical Society 134 (43) : 17972-17981. ScholarBank@NUS Repository.
Abstract: Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This fluorescence "turn-on" response is ascribed to aggregation of cleaved hydrophobic TPE residues, which restricts the intramolecular rotations of TPE phenyl rings and populates the radiative decay channels. The light-up nature of the probe allows real-time monitoring of caspase-3/-7 activities both in solutions and in living cells with a high signal-to-noise ratio. The probe provides a new opportunity to screen enzyme inhibitors and evaluate the apoptosis-associated drug efficacy. © 2012 American Chemical Society.
Source Title: Journal of the American Chemical Society
URI: http://scholarbank.nus.edu.sg/handle/10635/64490
ISSN: 00027863
Appears in Collections:Staff Publications

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