Please use this identifier to cite or link to this item: https://doi.org/10.1021/la104478x
Title: Probe-independent image correlation spectroscopy
Authors: Kurniawan, N.A.
Rajagopalan, R. 
Issue Date: 15-Mar-2011
Source: Kurniawan, N.A., Rajagopalan, R. (2011-03-15). Probe-independent image correlation spectroscopy. Langmuir 27 (6) : 2775-2782. ScholarBank@NUS Repository. https://doi.org/10.1021/la104478x
Abstract: Conventional image correlation spectroscopy (ICS) analysis assumes point-like probe particles whose sizes are much smaller than the beam focus. This assumption yields erroneous results when the particle size is larger than a certain threshold. Here, a formalism is presented to study image correlation spectroscopy for particles of arbitrary geometries, sizes, and fluorophore distributions. We demonstrate the usefulness of this method by analyzing simulated image sequences of diffusing fluorescent point sources, disks, and randomly oriented rigid rods of various sizes. In addition, we also perform ICS analysis on confocal images of fluorescent microspheres of different diameters diffusing in a medium of known viscosity to experimentally validate the method. The new method, which we call template analysis, yields excellent agreement with theoretical predictions, thus extending the capability of ICS for studying dynamic processes in a probe-independent manner. © 2011 American Chemical Society.
Source Title: Langmuir
URI: http://scholarbank.nus.edu.sg/handle/10635/64471
ISSN: 07437463
DOI: 10.1021/la104478x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

3
checked on Dec 5, 2017

WEB OF SCIENCETM
Citations

2
checked on Dec 5, 2017

Page view(s)

31
checked on Dec 18, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.