Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.actbio.2011.09.026
Title: PHBV microspheres as neural tissue engineering scaffold support neuronal cell growth and axon-dendrite polarization
Authors: Chen, W.
Tong, Y.W. 
Keywords: Microspheres
Neurons
PHBV
Tissue engineering
Issue Date: Feb-2012
Source: Chen, W., Tong, Y.W. (2012-02). PHBV microspheres as neural tissue engineering scaffold support neuronal cell growth and axon-dendrite polarization. Acta Biomaterialia 8 (2) : 540-548. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2011.09.026
Abstract: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microspheres, with properties such as slower degradation and more efficient drug delivery properties, have important benefits for neural tissue engineering. Our previous studies have shown PHBV microspheres to improve cell growth and differentiation. This study aimed to investigate if PHBV microspheres would support neurons to extend these benefits to neural tissue engineering. PHBV microspheres' suitability as neural tissue engineering scaffolds was investigated using PC12 cells, cortical neurons (CNs), and neural progenitor cells (NPCs) to cover a variety of neuronal types for different applications. Microspheres were fabricated using an emulsion-solvent-evaporation technique. DNA quantification, cell viability assays, and immunofluorescent staining were carried out. PC12 cultures on PHBV microspheres showed growth trends comparable to two-dimensional controls. This was further verified by staining for cell spreading. Also, CNs expressed components of the signaling pathway on PHBV microspheres, and had greater axon-dendrite segregation (4.1 times for axon stains and 2.3 times for dendrite stains) than on coverslips. NPCs were also found to differentiate into neurons on the microspheres. Overall, the results indicate that PHBV microspheres, as scaffolds for neural tissue engineering, supported a variety of neuronal cell types and promoted greater axon-dendrite segregation. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Source Title: Acta Biomaterialia
URI: http://scholarbank.nus.edu.sg/handle/10635/64399
ISSN: 17427061
DOI: 10.1016/j.actbio.2011.09.026
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

34
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

29
checked on Nov 21, 2017

Page view(s)

24
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.