Please use this identifier to cite or link to this item:
|Title:||Phalloidin-functionalized hyperbranched conjugated polyelectrolyte for filamentous actin imaging in living Hela cells|
fluorescence living cell imaging
hyperbranched conjugated polymer
|Citation:||Li, K., Pu, K.-Y., Cai, L., Liu, B. (2011-04-26). Phalloidin-functionalized hyperbranched conjugated polyelectrolyte for filamentous actin imaging in living Hela cells. Chemistry of Materials 23 (8) : 2113-2119. ScholarBank@NUS Repository. https://doi.org/10.1021/cm103557h|
|Abstract:||A phalloidin-functionalized hyperbranched conjugated polyelectrolyte (HCPE-phalloidin) is synthesized and used for direct filamentous actin (F-actin) imaging in living Hela cells. Different from commercially available organic dye-phalloidin conjugates, which require sophisticated techniques to be delivered into living cells, simple incubation of living cells with HCPE-phalloidin leads to efficient internalization of the probe and clear visualization of F-actin due to high brightness of HCPE and good specificity between phalloidin and actin. In addition, HCPE-phalloidin possesses improved photostability as compared to that for commercially available Alexa Fluor 488-phalloidin conjugates, suggesting that the new probe is promising for long-term F-actin imaging in living cells. Further fine-tuning the fluorescent property and targeting ability of HCPE-based probes could lead to more complicated imaging applications and subcellular target detection. © 2011 American Chemical Society.|
|Source Title:||Chemistry of Materials|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 25, 2018
WEB OF SCIENCETM
checked on Sep 18, 2018
checked on Sep 22, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.