Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2012.07.057
Title: Immobilization strategy for optimizing VEGF's concurrent bioactivity towards endothelial cells and osteoblasts on implant surfaces
Authors: Hu, X.
Neoh, K.G. 
Zhang, J.
Kang, E.-T. 
Wang, W.
Keywords: Bacterial adhesion
Endothelial cell
Heparin
Osteoblast
Protein conformation
VEGF immobilization
Issue Date: Nov-2012
Source: Hu, X., Neoh, K.G., Zhang, J., Kang, E.-T., Wang, W. (2012-11). Immobilization strategy for optimizing VEGF's concurrent bioactivity towards endothelial cells and osteoblasts on implant surfaces. Biomaterials 33 (32) : 8082-8093. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2012.07.057
Abstract: Orthopedic implant failure is mainly due to defective osseointegration and bacterial infection. Hence, a promising strategy to overcome these two problems is to functionalize the implant surface with both growth factors (GFs) and anti-infective agents. Covalent immobilization is widely used for such functionalization, but few studies have investigated the possible decrease in the GF's bioactivity as a result of conformational changes upon immobilization. In our study, vascular endothelial growth factor (VEGF) was immobilized on titanium surface via either covalent binding or heparin-VEGF interaction, and its bioactivity on endothelial cells (ECs) was compared. Although a similar surface density of immobilized VEGF was achieved by these two strategies, the bioactivity of the covalently immobilized VEGF on EC functions is significantly lower than that of the heparin-bound VEGF. The heparin-bound VEGF also enhanced mineralization in an osteoblast/endothelial cell co-culture to a much greater extent than in an osteoblast monoculture, illustrating the importance of crosstalk between osteoblasts and endothelial cells. In addition, the surface of the substrates with heparin-bound VEGF is highly hydrophilic and negatively-charged, which significantly inhibits Staphylococcus aureus adhesion. These results suggest that our strategy of immobilizing VEGF on titanium via heparin-VEGF interaction can preserve the GF's bioactivity on both osseous and vascular components and concomitantly reduce bacterial infection, which is promising to enhance the long-term stability of implants. © 2012 Elsevier Ltd.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/64060
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2012.07.057
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

35
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

35
checked on Dec 13, 2017

Page view(s)

32
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.