Please use this identifier to cite or link to this item:
https://doi.org/10.1002/marc.200900909
DC Field | Value | |
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dc.title | Homogeneous detection of trypsin in protein mixtures based on fluorescence resonance energy transfer between anionic conjugated polymer and fluorescent probe | |
dc.contributor.author | Zhu, O. | |
dc.contributor.author | Zhan, R. | |
dc.contributor.author | Liu, B. | |
dc.date.accessioned | 2014-06-17T07:42:31Z | |
dc.date.available | 2014-06-17T07:42:31Z | |
dc.date.issued | 2010-06-16 | |
dc.identifier.citation | Zhu, O., Zhan, R., Liu, B. (2010-06-16). Homogeneous detection of trypsin in protein mixtures based on fluorescence resonance energy transfer between anionic conjugated polymer and fluorescent probe. Macromolecular Rapid Communications 31 (12) : 1060-1064. ScholarBank@NUS Repository. https://doi.org/10.1002/marc.200900909 | |
dc.identifier.issn | 10221336 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/64037 | |
dc.description.abstract | Herein we describe a novel and simple conjugated polymer-fluorescent probe based platform for trypsin detection from protein mixtures in homogeneous solution. This platform takes advantage of specific interaction between the probe and the active site of trypsin and the electrostatic interaction between the polymer and the protein to mediate energy transfer between the polymer and the probe. This method does not require any separation steps, which should facilitate high-throughput protease screening and drug discovery. (Figure Presented) © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/marc.200900909 | |
dc.source | Scopus | |
dc.subject | Conjugated polyelectrolyte | |
dc.subject | Conjugated polymers | |
dc.subject | Fluorescence | |
dc.subject | Fluorescence resonance energy transfer (fret) | |
dc.subject | Fluorescent probes | |
dc.subject | Protein detection | |
dc.subject | Trypsin | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/marc.200900909 | |
dc.description.sourcetitle | Macromolecular Rapid Communications | |
dc.description.volume | 31 | |
dc.description.issue | 12 | |
dc.description.page | 1060-1064 | |
dc.description.coden | MRCOE | |
dc.identifier.isiut | 000279456700002 | |
Appears in Collections: | Staff Publications |
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