Please use this identifier to cite or link to this item: https://doi.org/10.1002/marc.200900909
Title: Homogeneous detection of trypsin in protein mixtures based on fluorescence resonance energy transfer between anionic conjugated polymer and fluorescent probe
Authors: Zhu, O.
Zhan, R.
Liu, B. 
Keywords: Conjugated polyelectrolyte
Conjugated polymers
Fluorescence
Fluorescence resonance energy transfer (fret)
Fluorescent probes
Protein detection
Trypsin
Issue Date: 16-Jun-2010
Source: Zhu, O., Zhan, R., Liu, B. (2010-06-16). Homogeneous detection of trypsin in protein mixtures based on fluorescence resonance energy transfer between anionic conjugated polymer and fluorescent probe. Macromolecular Rapid Communications 31 (12) : 1060-1064. ScholarBank@NUS Repository. https://doi.org/10.1002/marc.200900909
Abstract: Herein we describe a novel and simple conjugated polymer-fluorescent probe based platform for trypsin detection from protein mixtures in homogeneous solution. This platform takes advantage of specific interaction between the probe and the active site of trypsin and the electrostatic interaction between the polymer and the protein to mediate energy transfer between the polymer and the probe. This method does not require any separation steps, which should facilitate high-throughput protease screening and drug discovery. (Figure Presented) © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Macromolecular Rapid Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/64037
ISSN: 10221336
DOI: 10.1002/marc.200900909
Appears in Collections:Staff Publications

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