Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.b.30698
Title: Growing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microspheres of different sizes
Authors: Xin, H.Z. 
Wang, C.-H. 
Tong, Y.W. 
Keywords: Liver cells
Liver tissue engineering
Microspheres
PHBV
Issue Date: Jul-2007
Source: Xin, H.Z., Wang, C.-H., Tong, Y.W. (2007-07). Growing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microspheres of different sizes. Journal of Biomedical Materials Research - Part B Applied Biomaterials 82 (1) : 7-16. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.b.30698
Abstract: In this study, an oil-in-water emulsion solvent evaporation technique was used to fabricate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV, 8% PHV), microspheres as scaffold, to guide liver cell growth. Human hepatoma cell lines, HepG2 and Hep3B, were cultured in vitro on both the microspheres and polymer films. SEM and optical microscope images showed that multilayer cells were formed among the microspheres to bridge them together and developed into cell-construct aggregates after 1 week of culture. MTT results showed that the cell proliferation on the microspheres was more than two times higher than that on the films after 12 days of culture. The cells seeded on microspheres secreted albumin 2-4 times more than that on the positive control after 1 week of culture, which indicated that this hepatic function was greatly improved by the aggregation of cells on microspheres. Although HepG2 failed to express P-450 activity, this hepatic function was preserved when Hep3B cultured on microspheres. All the results indicated that PHBV microspheres are appropriate scaffolds for liver tissue engineering. © 2006 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part B Applied Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/63999
ISSN: 15524973
DOI: 10.1002/jbm.b.30698
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