Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.21924
Title: Formulation, characterization, and in vitro evaluation of quantum dots loaded in poly(Lactide)-Vitamin E TPGS nanoparticles for cellular and molecular imaging
Authors: Pan, J.
Wang, Y.
Feng, S.-S. 
Keywords: Biodegradable polymers
Cancer nanotechnology
Fluorescence
Nanobiotechnology
Nanomedicine
Issue Date: 15-Oct-2008
Citation: Pan, J., Wang, Y., Feng, S.-S. (2008-10-15). Formulation, characterization, and in vitro evaluation of quantum dots loaded in poly(Lactide)-Vitamin E TPGS nanoparticles for cellular and molecular imaging. Biotechnology and Bioengineering 101 (3) : 622-633. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.21924
Abstract: We developed a novel system of polyflactide acid)-d-α-tocopheryl polyethylene glycol 1000 succinate (PLA-TPGS) nanoparticles (NPs) for quantum dots (QDs) formulation to improve imaging effects and reduce side effects as well as to promote a sustainable imaging. The QDs-loaded PLA-TPGS NPs were prepared by a modified solvent extraction/evaporation method, which were then characterized by laser light scattering (LLS) for size and size distribution; field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and transmission electron microscope (TEM) for surface morphology. Surface chemistry of the QDs-loaded PLA-TPGS NPs was analyzed by X-ray photoelectron microscopy (XPS) and Fourier transform infra-red spectroscopy (FTIR). Encapsulation efficiency of the QDs in the polymeric nanoparticles was measured by inductively coupled plasma optical emission spectrometry (ICP-OES). The photostability of the QDs formulated in the PLA-TPGS nanoparticles was investigated as changes in the florescence intensity versus the irradiation time. Confocal laser scanning microscopy (CLSM) was used to image the cellular uptake of the QDs-loaded NPs by MCF-7 cells. Methylthiazolyldiphenyl-tetrazolium (MTT) assay was employed to assess the viability of MCF-7 cells incubated with the QDs formulated by the PLA-TPGS NPs versus the mercaptoacetic acid (MAA)-coated QDs. It was found that the QDs formulated in the PLA-TPGS NPs can result in higher fluorescence intensity and higher photo-stability than the bare QDs as well as lower cytotoxicity than the MAA-coated QDs. © 2008 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
URI: http://scholarbank.nus.edu.sg/handle/10635/63948
ISSN: 00063592
DOI: 10.1002/bit.21924
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