Please use this identifier to cite or link to this item:
Title: Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte
Authors: Shi, H.
Wang, G.
Liang, J.
Liu, B. 
Issue Date: Mar-2013
Citation: Shi, H., Wang, G., Liang, J., Liu, B. (2013-03). Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte. MedChemComm 4 (3) : 554-558. ScholarBank@NUS Repository.
Abstract: A fluorescence "turn-on" assay for live cell apoptosis imaging is developed based on self-assembled complexes between a cationic water-soluble hyperbranched conjugated polyelectrolyte (HCPE) and an Asp-Glu-Val-Asp (DEVD) peptide substrate labelled with a fluorescence quencher (DEVD-dabcyl). The polymer fluorescence can be significantly quenched upon DEVD-dabcyl binding, and the fluorescence is switched on upon treatment of the complexes with caspase-3/7, which are activated in the cell apoptotic process and are able to cleave the DEVD moiety. The light-up nature of the assay allows monitoring of caspase-3/7 activities both in solutions and in living cells with a high signal to noise ratio, which provides a new opportunity to screen caspase inhibitors and detect cell apoptosis. © 2013 The Royal Society of Chemistry.
Source Title: MedChemComm
ISSN: 20402503
DOI: 10.1039/c2md20240a
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 14, 2019


checked on Jan 7, 2019

Page view(s)

checked on Jan 12, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.