Please use this identifier to cite or link to this item:
|Title:||Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte|
|Source:||Shi, H., Wang, G., Liang, J., Liu, B. (2013-03). Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte. MedChemComm 4 (3) : 554-558. ScholarBank@NUS Repository. https://doi.org/10.1039/c2md20240a|
|Abstract:||A fluorescence "turn-on" assay for live cell apoptosis imaging is developed based on self-assembled complexes between a cationic water-soluble hyperbranched conjugated polyelectrolyte (HCPE) and an Asp-Glu-Val-Asp (DEVD) peptide substrate labelled with a fluorescence quencher (DEVD-dabcyl). The polymer fluorescence can be significantly quenched upon DEVD-dabcyl binding, and the fluorescence is switched on upon treatment of the complexes with caspase-3/7, which are activated in the cell apoptotic process and are able to cleave the DEVD moiety. The light-up nature of the assay allows monitoring of caspase-3/7 activities both in solutions and in living cells with a high signal to noise ratio, which provides a new opportunity to screen caspase inhibitors and detect cell apoptosis. © 2013 The Royal Society of Chemistry.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 6, 2017
WEB OF SCIENCETM
checked on Nov 19, 2017
checked on Dec 10, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.