Please use this identifier to cite or link to this item: https://doi.org/10.1002/adma.200801803
Title: Exploring optical properties of liquid crystals for developing label-free and high-throughput microfluidic immunoassays
Authors: Xue, C.-Y.
Khan, S.A. 
Yang, K.-L. 
Issue Date: 12-Jan-2009
Source: Xue, C.-Y., Khan, S.A., Yang, K.-L. (2009-01-12). Exploring optical properties of liquid crystals for developing label-free and high-throughput microfluidic immunoassays. Advanced Materials 21 (2) : 198-202. ScholarBank@NUS Repository. https://doi.org/10.1002/adma.200801803
Abstract: A liquid crystals (LC)-based detecting method suitable for microfluidic immunoassays was successfully demonstrated in the study. The microfluidic device comprised three serpentine channels supported on an immunoglobulin (IgG)-coated glass slides and sealed with poly(dimethylsiloxane) (PDMS) with embedded microfluidic channels. Two fluorescence-labeled antibody solutions, fluorescein-isothiocyanate-conjugated anti-human IgG (FITC-anti0IgG) and FITC-conjugated anti-biotin IgG (FITC-anti-biotin), were then piped into the inlet reservoirs of two separate channels, allowing both solutions to enter the microfluidic channels by capillary actions. After 30 minutes, the slides were rinsed with PBS buffer with 1% SDS and deionized water. It was demonstrated that LC-based detection and microfluidic immunoassays provide a label-free, multiplexed and high-throughput diagnostic platform, which can be used to detect multiple samples of anti-IgG, anti-biotin and mixture of both.
Source Title: Advanced Materials
URI: http://scholarbank.nus.edu.sg/handle/10635/63895
ISSN: 09359648
DOI: 10.1002/adma.200801803
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

37
checked on Dec 11, 2017

WEB OF SCIENCETM
Citations

36
checked on Dec 11, 2017

Page view(s)

27
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.