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|Title:||Enhancing the fluorescence intensity of DNA microarrays by using cationic surfactants|
|Citation:||Lai, S.L., Chen, C.-H., Yang, K.-L. (2011-05-03). Enhancing the fluorescence intensity of DNA microarrays by using cationic surfactants. Langmuir 27 (9) : 5659-5664. ScholarBank@NUS Repository. https://doi.org/10.1021/la2004694|
|Abstract:||DNA microarrays have been used as powerful tools in genomics studies and single nucleotide polymorphisms analysis. However, the fluorescence detection used in most conventional DNA microarrays is still limited by its sensitivity. The aim of this study is to use a cationic surfactant, cetyl trimethylammonium bromide (CTAB), to enhance the fluorescence intensity of 6-carboxy-fluorescene (FAM)-labeled DNA probes immobilized on a DNA microarray. We show that in the presence of CTAB the immobilized FAM-labeled DNA probes is 11-fold brighter than that without exposure to CTAB. Similarly, when we hybridize FAM-labeled DNA targets to a DNA microarray and treat the surface with CTAB solution, the fluorescence intensity shows a 26-fold increase for perfect-match DNA targets. More importantly, the contrast between perfect-match and 1-mismatch DNA is also increased from 1.3-fold to 15-fold. This method offers a simple and efficient technique to enhance the detection limit of DNA microarrays. © 2011 American Chemical Society.|
|Appears in Collections:||Staff Publications|
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