Please use this identifier to cite or link to this item:
https://doi.org/10.1080/09205063.2012.703949
Title: | Enhancing bioactivity of chitosan film for osteogenesis and wound healing by covalent immobilization of BMP-2 or FGF-2 |
Authors: | Budiraharjo, R. Neoh, K.G. Kang, E.-T. |
Keywords: | Bioactivity BMP-2 Chitosan film Covalent immobilization FGF-2 |
Issue Date: | Apr-2013 |
Citation: | Budiraharjo, R., Neoh, K.G., Kang, E.-T. (2013-04). Enhancing bioactivity of chitosan film for osteogenesis and wound healing by covalent immobilization of BMP-2 or FGF-2. Journal of Biomaterials Science, Polymer Edition 24 (6) : 645-662. ScholarBank@NUS Repository. https://doi.org/10.1080/09205063.2012.703949 |
Abstract: | This study compares the efficacy of growth factors that are covalently immobilized to those that are adsorbed in improving the bioactivity of a biomaterial. Bone morphogenetic protein-2 (BMP-2) or fibroblast growth factor-2 (FGF-2) was covalently bonded to chitosan films using carbodiimide chemistry. For BMP-2, a growth factor loading efficiency of ∼64% was obtained with this method compared to ∼25% from adsorption. As for FGF-2, the growth factor loading efficiency of the two methods was similar at ∼50%. The covalently immobilized BMP-2 promoted attachment, proliferation, and differentiation of osteoblasts in a dose-dependent manner, whereas the covalently immobilized FGF-2 stimulated fibroblast attachment, proliferation, and collagen synthesis. After three weeks immersion in phosphate buffered saline, about 80% of the covalently immobilized growth factors were retained on the films, while only ∼16 and ∼21% of the adsorbed BMP-2 and FGF-2 remained on the corresponding films. The higher retention rate of the covalently immobilized growth factors enabled their stimulatory effects to persist for a longer period than when adsorbed growth factors were used. |
Source Title: | Journal of Biomaterials Science, Polymer Edition |
URI: | http://scholarbank.nus.edu.sg/handle/10635/63848 |
ISSN: | 09205063 |
DOI: | 10.1080/09205063.2012.703949 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
SCOPUSTM
Citations
20
checked on Feb 18, 2019
WEB OF SCIENCETM
Citations
19
checked on Feb 18, 2019
Page view(s)
39
checked on Dec 29, 2018
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.