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|Title:||Enhancing bioactivity of chitosan film for osteogenesis and wound healing by covalent immobilization of BMP-2 or FGF-2|
|Citation:||Budiraharjo, R., Neoh, K.G., Kang, E.-T. (2013-04). Enhancing bioactivity of chitosan film for osteogenesis and wound healing by covalent immobilization of BMP-2 or FGF-2. Journal of Biomaterials Science, Polymer Edition 24 (6) : 645-662. ScholarBank@NUS Repository. https://doi.org/10.1080/09205063.2012.703949|
|Abstract:||This study compares the efficacy of growth factors that are covalently immobilized to those that are adsorbed in improving the bioactivity of a biomaterial. Bone morphogenetic protein-2 (BMP-2) or fibroblast growth factor-2 (FGF-2) was covalently bonded to chitosan films using carbodiimide chemistry. For BMP-2, a growth factor loading efficiency of ∼64% was obtained with this method compared to ∼25% from adsorption. As for FGF-2, the growth factor loading efficiency of the two methods was similar at ∼50%. The covalently immobilized BMP-2 promoted attachment, proliferation, and differentiation of osteoblasts in a dose-dependent manner, whereas the covalently immobilized FGF-2 stimulated fibroblast attachment, proliferation, and collagen synthesis. After three weeks immersion in phosphate buffered saline, about 80% of the covalently immobilized growth factors were retained on the films, while only ∼16 and ∼21% of the adsorbed BMP-2 and FGF-2 remained on the corresponding films. The higher retention rate of the covalently immobilized growth factors enabled their stimulatory effects to persist for a longer period than when adsorbed growth factors were used.|
|Source Title:||Journal of Biomaterials Science, Polymer Edition|
|Appears in Collections:||Staff Publications|
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