Please use this identifier to cite or link to this item:
Title: Elucidation of metabolism in hybridoma cells grown in fed-batch culture by genome-scale modeling
Authors: Selvarasu, S.
Wong, V.V.T.
Karimi, I.A. 
Lee, D.-Y. 
Keywords: Constraints-based flux analysis
Genome scale metabolic network
Human IgG1
Mouse hybridoma cells
Mus musculus
Issue Date: 1-Apr-2009
Source: Selvarasu, S., Wong, V.V.T., Karimi, I.A., Lee, D.-Y. (2009-04-01). Elucidation of metabolism in hybridoma cells grown in fed-batch culture by genome-scale modeling. Biotechnology and Bioengineering 102 (5) : 1494-1504. ScholarBank@NUS Repository.
Abstract: Genome-scale modeling of mouse hybridoma cells producing monoclonal antibodies (mAb) was performed to elucidate their physiological and metabolic states during fed-batch cell culture. Initially, feed media nutrients were monitored to identify key components among carbon sources and amino acids with significant impact on the desired outcome, for example, cell growth and antibody production. The monitored profiles indicated rapid assimilation of glucose and glutamine during the exponential growth phase. Significant increase in mAb concentration was also observed when glutamine concentration was controlled at 0.5 mM as a feeding strategy. Based on the reconstructed genome-scale metabolic network of mouse hybridoma cells and fed-batch profiles, flux analysis was then implemented to investigate the cellular behavior and changes in internal fluxes during the cell culture. The simulated profile of the cell growth was consistent with experimentally measured specific growth rate. The in silico simulation results indicated (i) predominant utilization of glycolytic pathway for ATP production, (ii) importance of pyruvate node in metabolic shifting, and (iii) characteristic pattern in lactate to glucose ratio during the exponential phase. In future, experimental and in silico analyses can serve as a promising approach to identifying optimal feeding strategies and potential cell engineering targets as well as facilitate media optimization for the enhanced production of mAb or recombinant proteins in mammalian cells. ©2008 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
ISSN: 00063592
DOI: 10.1002/bit.22186
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 7, 2018


checked on Jan 31, 2018

Page view(s)

checked on Mar 11, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.