Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.12.053
Title: Efficient transformation of grease to biodiesel using highly active and easily recyclable magnetic nanobiocatalyst aggregates
Authors: Ngo, T.P.N.
Li, A. 
Tiew, K.W.
Li, Z. 
Keywords: Biodiesel
Enzyme catalysis
Free fatty acid
Grease
Magnetic nanoparticles
Issue Date: Oct-2013
Source: Ngo, T.P.N., Li, A., Tiew, K.W., Li, Z. (2013-10). Efficient transformation of grease to biodiesel using highly active and easily recyclable magnetic nanobiocatalyst aggregates. Bioresource Technology 145 : 233-239. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.12.053
Abstract: Green and efficient production of biodiesel (FAME) from waste grease containing high amount of free fatty acid (FFA) was achieved by using novel magnetic nanobiocatalyst aggregates (MNA). Thermomyces lanuginosus Lipase (TLL) and Candida antartica Lipase B (CALB) were covalently immobilized on core-shell structured iron oxide magnetic nanoparticle (80. nm), respectively, followed by freeze-dry to give MNA (13-17. μm) with high yield (80-89%) and high enzyme loading (61. mg TLL or 22. mg CALB per gram MNA). MNA TL showed the best performance among immobilized enzymes known thus for the production of FAME from grease (17. wt.% FFA) with methanol, giving 99% yield in 12. h (3.3. wt.% catalyst). MNA TL was easily separated under magnetic field and reused, retaining 88% productivity in 11th cycle. MNA CA converted >97% FFA in grease (17. wt.% FFA) to FAME in 12. h (0.45. wt.% catalyst), being useful in two-step transformation of grease to biodiesel. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/63802
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.12.053
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