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|Title:||Efficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases|
Free fatty acid
|Citation:||Yan, J., Li, A., Xu, Y., Ngo, T.P.N., Phua, S., Li, Z. (2012-11). Efficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases. Bioresource Technology 123 : 332-337. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.103|
|Abstract:||A novel concept and efficient method for producing biodiesel (FAME) from grease (15-40. wt% free fatty acid, FFA) were developed by using tandem lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system. Combining immobilized Candida antarctica lipase B (CALB) (Novozyme 435) favoring the esterification and immobilized Thermomyces lanuginosus lipase (TLL) (Lipozyme TLIM) preferring the transesterification at 2:8 (wt/wt) gave FAME in 80% yield, being better than that with Novozyme 435 or Lipozyme TLIM. Recombinant Escherichia coli (Calb/Tll) co-expressing CALB and TLL was engineered as a more efficient tandem-lipases system. Using wet or dry cells (4. wt%) gave FAME in 87% or 95% yield, which is much better than that with E. coli cells expressing either CALB or TLL alone. Cells of E. coli (Calb/Tll) were recycled for five times and retained 75% productivity, thus being practical for producing biodiesel from grease. © 2012 Elsevier Ltd.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
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