Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.07.103
Title: Efficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases
Authors: Yan, J.
Li, A. 
Xu, Y. 
Ngo, T.P.N.
Phua, S.
Li, Z. 
Keywords: Biodiesel
Free fatty acid
Grease
Tandem enzymes
Whole-cells catalysis
Issue Date: Nov-2012
Source: Yan, J., Li, A., Xu, Y., Ngo, T.P.N., Phua, S., Li, Z. (2012-11). Efficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases. Bioresource Technology 123 : 332-337. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.103
Abstract: A novel concept and efficient method for producing biodiesel (FAME) from grease (15-40. wt% free fatty acid, FFA) were developed by using tandem lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system. Combining immobilized Candida antarctica lipase B (CALB) (Novozyme 435) favoring the esterification and immobilized Thermomyces lanuginosus lipase (TLL) (Lipozyme TLIM) preferring the transesterification at 2:8 (wt/wt) gave FAME in 80% yield, being better than that with Novozyme 435 or Lipozyme TLIM. Recombinant Escherichia coli (Calb/Tll) co-expressing CALB and TLL was engineered as a more efficient tandem-lipases system. Using wet or dry cells (4. wt%) gave FAME in 87% or 95% yield, which is much better than that with E. coli cells expressing either CALB or TLL alone. Cells of E. coli (Calb/Tll) were recycled for five times and retained 75% productivity, thus being practical for producing biodiesel from grease. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/63801
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.07.103
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

35
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

30
checked on Nov 21, 2017

Page view(s)

40
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.