Please use this identifier to cite or link to this item: https://doi.org/10.1080/02652040410001729214
Title: Effects of polymer, organic solvent and mixing strength on integrity of proteins and liposomes encapsulated in polymeric microspheres fabricated by the double emulsion process
Authors: Ruan, G.
Ng, J.-K.
Feng, S.-S. 
Keywords: Drug delivery
Integrity
Liposomes
Sonication
W/o emulsion
Issue Date: Jun-2004
Source: Ruan, G., Ng, J.-K., Feng, S.-S. (2004-06). Effects of polymer, organic solvent and mixing strength on integrity of proteins and liposomes encapsulated in polymeric microspheres fabricated by the double emulsion process. Journal of Microencapsulation 21 (4) : 399-412. ScholarBank@NUS Repository. https://doi.org/10.1080/02652040410001729214
Abstract: The double emulsion process has commonly been applied to encapsulate water-soluble bioactive agents into polymeric microspheres. However, the integrity of many of these agents may be destroyed by the highly energetic procedures such as sonication that are routinely used to produce stable water-in-oil (w/o) emulsion. The aim of this research was to pursue the possibility of replacing the sonication by a mild emulsification procedure such as vortex mixing, with the use of certain materials to help to obtain stable w/o emulsion. The following materials were examined: poly(lactide-co-ethylene glycol) (PELA) as the polymer, ethyl acetate and acetone as the solvents, poly(vinyl alcohol) (PVA) and d-α tocopheryl polyethylene glycol 1000 succinate (Vitamin E TPGS) as the emulsifiers in w/o emulsion. The experimental results, with human serum albumin (HSA) as the encapsulated agent, showed that, when vortex mixing was used, these materials could significantly improve w/o emulsion stability and help to obtain satisfactory encapsulation effects, i.e. high encapsulation efficiency (EE) and low initial release burst. A delicate structure, i.e. liposomes, which is very sensitive to sonication, was then incorporated into microspheres by the 'modified double emulsion process'. It was found that the liposomes were intact and the encapsulation effects were good. Therefore, it can be concluded that the modified double emulsion process could be advantageous for the encapsulation of delicate substances. © 2004 Taylor & Francis Ltd.
Source Title: Journal of Microencapsulation
URI: http://scholarbank.nus.edu.sg/handle/10635/63790
ISSN: 02652048
DOI: 10.1080/02652040410001729214
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

18
checked on Dec 14, 2017

WEB OF SCIENCETM
Citations

14
checked on Nov 18, 2017

Page view(s)

26
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.