Please use this identifier to cite or link to this item:
|Title:||Effects of macromolecular crowding on biochemical reaction equilibria: Amolecular thermodynamic perspective|
|Citation:||Hu, Z., Jiang, J., Rajagopalan, R. (2007-09). Effects of macromolecular crowding on biochemical reaction equilibria: Amolecular thermodynamic perspective. Biophysical Journal 93 (5) : 1464-1473. ScholarBank@NUS Repository. https://doi.org/10.1529/biophysj.107.104646|
|Abstract:||A molecular thermodynamic model is developed to investigate the effects of macromolecular crowding on biochemical reactions. Three types of reactions, representing protein folding/conformational isomerization, coagulation/ coalescence, and polymerization/association, are considered. The reactants, products, and crowders are modeled as coarse-grained spherical particles or as polymer chains, interacting through hard-sphere interactions with or without nonbonded square-well interactions, and the effects of crowder size and chain length as well as product size are examined. The results predicted by this model are consistent with experimentally observed crowding effects based on preferential binding or preferential exclusion of the crowders. Although simple hard-core excluded-volume arguments do in general predict the qualitative aspects of the crowding effects, the results show that other intermolecular interactions can substantially alter the extent of enhancement or reduction of the equilibrium and can even change the direction of the shift. An advantage of the approach presented here is that competing reactions can be incorporated within the model. © 2007 by the Biophysical Society.|
|Source Title:||Biophysical Journal|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 17, 2018
WEB OF SCIENCETM
checked on Jun 4, 2018
checked on Jun 1, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.