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Title: Conversion of acid hydrolysate of oil palm empty fruit bunch to L-lactic acid by newly isolated Bacillus coagulans JI12
Authors: Ye, L.
Hudari, M.S.B.
Zhou, X.
Zhang, D.
Li, Z. 
Wu, J.C.
Keywords: Bacillus coagulans
Empty fruit bunch of oil palm (EFB)
L-lactic acid
Issue Date: Jun-2013
Citation: Ye, L., Hudari, M.S.B., Zhou, X., Zhang, D., Li, Z., Wu, J.C. (2013-06). Conversion of acid hydrolysate of oil palm empty fruit bunch to L-lactic acid by newly isolated Bacillus coagulans JI12. Applied Microbiology and Biotechnology 97 (11) : 4831-4838. ScholarBank@NUS Repository.
Abstract: Cost-effective conversion of lignocellulose hydrolysate to optically pure lactic acid is commercially attractive but very challenging. Bacillus coagulans JI12 was isolated from natural environment and used to produce L-lactic acid (optical purity > 99.5 %) from lignocellulose sugars and acid hydrolysate of oil palm empty fruit bunch (EFB) at 50 °C and pH 6.0 without sterilization of the medium. In fed-batch fermentation with 85 g/L initial xylose and 55 g/L xylose added after 7.5 h, 137.5 g/L lactic acid was produced with a yield of 98 % and a productivity of 4.4 g/L h. In batch fermentation of a sugar mixture containing 8.5 % xylose, 1 % glucose, and 1 % L-arabinose, the lactic acid yield and productivity reached 98 % and 4.8 g/L h, respectively. When EFB hydrolysate was used, 59.2 g/L of lactic acid was produced within 9.5 h at a yield of 97 % and a productivity of 6.2 g/L h, which are the highest among those ever reported from lignocellulose hydrolysates. These results indicate that B. coagulans JI12 is a promising strain for industrial production of L-lactic acid from lignocellulose hydrolysate. © 2013 Springer-Verlag Berlin Heidelberg.
Source Title: Applied Microbiology and Biotechnology
ISSN: 01757598
DOI: 10.1007/s00253-013-4788-y
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