Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac102949u
Title: Conjugated polymer loaded nanospheres with surface functionalization for simultaneous discrimination of different live cancer cells under single wavelength excitation
Authors: Li, K.
Zhan, R.
Feng, S.-S. 
Liu, B. 
Issue Date: 15-Mar-2011
Source: Li, K., Zhan, R., Feng, S.-S., Liu, B. (2011-03-15). Conjugated polymer loaded nanospheres with surface functionalization for simultaneous discrimination of different live cancer cells under single wavelength excitation. Analytical Chemistry 83 (6) : 2125-2132. ScholarBank@NUS Repository. https://doi.org/10.1021/ac102949u
Abstract: Two conjugated polymers, poly[9,9-bis(2-(2-(2- methoxyethoxy)ethoxy)ethyl) fluorenyldivinylene] (PFV) and the PFV derivative containing 10 mol % 2,1,3-benzothiadiazole (BT) units (PFVBT), have been synthesized and employed to fabricate conjugated polymer loaded nanospheres for simultaneous discrimination of mixed live cancer cells in one solution. The incorporation of BT units into the PFV backbone leads to PFVBT with a similar absorption maximum but significantly redshifted emission in film state as compared to those of PFV, due to aggregation enhanced energy transfer from the fluorenevinylene segments to electron-deficient BT units. Both conjugated polymer loaded nanospheres have shown optical features that are similar to their film states, which allow simultaneous multichannel signal collection with negligible interference upon excitation at a single wavelength. After further surface functionalization with antihuman epidermal growth factor receptor 2 (HER2) affibody or arginine-glycine-aspartic acid (RGD) peptide, the distinct fluorescence from PFV or PFVBT loaded nanospheres allows differentiation of SKBR-3 breast cancer cells (HER2 overexpression) from HT-29 colon cancer cells (integrin receptor overexpression) in live cell mixtures. The conjugated polymer loaded nanospheres with high quantum yield, low cytotoxicity, and multiple color emission upon single laser excitation are ideal for simultaneous multiple-target imaging and detection. © 2011 American Chemical Society.
Source Title: Analytical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/63648
ISSN: 00032700
DOI: 10.1021/ac102949u
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