Please use this identifier to cite or link to this item:
|Title:||Conjugated polyelectrolyte as signal amplifier for fluorogenic probe based enzyme activity study|
|Source:||Zhan, R., Tan, A.J.H., Liu, B. (2011-02). Conjugated polyelectrolyte as signal amplifier for fluorogenic probe based enzyme activity study. Polymer Chemistry 2 (2) : 417-421. ScholarBank@NUS Repository. https://doi.org/10.1039/c0py00265h|
|Abstract:||A cationic conjugated polyelectrolyte (CPE) is used as a signal amplifier to enhance the sensory response of a fluorogenic substrate (fluorescein diacetate) for esterase activity study. As fluorescein diacetate is nonfluorescent, it can be cleaved by esterase to yield a negatively charged fluorescent dye. Electrostatic interaction between the cationic CPE and negatively charged fluorescent dye leads to complexes which allow fluorescence resonance energy transfer to amplify the dye signal by a factor of up to ∼19-fold. The presence of CPE does not obviously influence the enzyme efficiency and the assay allows real-time monitoring of enzyme activity. As compared with common fluorogenic assays, the CPE amplified system has the advantage of shorter readout time with higher sensitivity, which could have wide applications in high throughput screening of enzyme activity. © 2011 The Royal Society of Chemistry.|
|Source Title:||Polymer Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 14, 2017
WEB OF SCIENCETM
checked on Nov 17, 2017
checked on Dec 10, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.