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https://doi.org/10.1002/jor.21409
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dc.title | Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth | |
dc.contributor.author | Poh, C.K. | |
dc.contributor.author | Shi, Z. | |
dc.contributor.author | Tan, X.W. | |
dc.contributor.author | Liang, Z.C. | |
dc.contributor.author | Foo, X.M. | |
dc.contributor.author | Tan, H.C. | |
dc.contributor.author | Neoh, K.G. | |
dc.contributor.author | Wang, W. | |
dc.date.accessioned | 2014-06-17T07:37:20Z | |
dc.date.available | 2014-06-17T07:37:20Z | |
dc.date.issued | 2011-09 | |
dc.identifier.citation | Poh, C.K., Shi, Z., Tan, X.W., Liang, Z.C., Foo, X.M., Tan, H.C., Neoh, K.G., Wang, W. (2011-09). Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth. Journal of Orthopaedic Research 29 (9) : 1424-1430. ScholarBank@NUS Repository. https://doi.org/10.1002/jor.21409 | |
dc.identifier.issn | 07360266 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/63597 | |
dc.description.abstract | Cobalt chromium (CoCr) alloys are widely used in orthopedic practice, however, lack of integration into the bone for long-term survival often occurs, leading to implant failure. Revision surgery to address such a failure involves increased risks, complications, and costs. Advances to enhancement of bone-implant interactions would improve implant longevity and long-term results. Therefore, we investigated the effects of BMP peptide covalently grafted to CoCr alloy on osteogenesis. The BMP peptide was derived from the knuckle epitope of bone morphogenic protein-2 (BMP-2) and was conjugated via a cysteine amino acid at the N-terminus. X-ray photoelectron spectroscopy and o-phthaldialdehyde were used to verify successful grafting at various stages of surface functionalization. Surface topography was evaluated from the surface profile determined by atomic force microscopy. Osteoblastic cells (MC3T3-E1) were seeded on the substrates, and the effects of BMP peptide on osteogenic differentiation were evaluated by measuring alkaline phosphatase (ALP) activity and calcium mineral deposition. The functionalized surfaces showed a twofold increase in ALP activity after 2 weeks incubation and a fourfold increase in calcium content after 3 weeks incubation compared to the pristine substrate. These findings are potentially useful in the development of improved CoCr implants for use in orthopedic applications. © 2011 Orthopaedic Research Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jor.21409 | |
dc.source | Scopus | |
dc.subject | BMP peptide | |
dc.subject | cobalt chromium | |
dc.subject | orthopedic implants | |
dc.subject | surface modification | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/jor.21409 | |
dc.description.sourcetitle | Journal of Orthopaedic Research | |
dc.description.volume | 29 | |
dc.description.issue | 9 | |
dc.description.page | 1424-1430 | |
dc.description.coden | JORED | |
dc.identifier.isiut | 000293735800017 | |
Appears in Collections: | Staff Publications |
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