Please use this identifier to cite or link to this item:
Title: Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth
Authors: Poh, C.K.
Shi, Z. 
Tan, X.W.
Liang, Z.C.
Foo, X.M.
Tan, H.C.
Neoh, K.G. 
Wang, W.
Keywords: BMP peptide
cobalt chromium
orthopedic implants
surface modification
Issue Date: Sep-2011
Source: Poh, C.K., Shi, Z., Tan, X.W., Liang, Z.C., Foo, X.M., Tan, H.C., Neoh, K.G., Wang, W. (2011-09). Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth. Journal of Orthopaedic Research 29 (9) : 1424-1430. ScholarBank@NUS Repository.
Abstract: Cobalt chromium (CoCr) alloys are widely used in orthopedic practice, however, lack of integration into the bone for long-term survival often occurs, leading to implant failure. Revision surgery to address such a failure involves increased risks, complications, and costs. Advances to enhancement of bone-implant interactions would improve implant longevity and long-term results. Therefore, we investigated the effects of BMP peptide covalently grafted to CoCr alloy on osteogenesis. The BMP peptide was derived from the knuckle epitope of bone morphogenic protein-2 (BMP-2) and was conjugated via a cysteine amino acid at the N-terminus. X-ray photoelectron spectroscopy and o-phthaldialdehyde were used to verify successful grafting at various stages of surface functionalization. Surface topography was evaluated from the surface profile determined by atomic force microscopy. Osteoblastic cells (MC3T3-E1) were seeded on the substrates, and the effects of BMP peptide on osteogenic differentiation were evaluated by measuring alkaline phosphatase (ALP) activity and calcium mineral deposition. The functionalized surfaces showed a twofold increase in ALP activity after 2 weeks incubation and a fourfold increase in calcium content after 3 weeks incubation compared to the pristine substrate. These findings are potentially useful in the development of improved CoCr implants for use in orthopedic applications. © 2011 Orthopaedic Research Society.
Source Title: Journal of Orthopaedic Research
ISSN: 07360266
DOI: 10.1002/jor.21409
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 13, 2018


checked on Mar 13, 2018

Page view(s)

checked on Mar 11, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.