Please use this identifier to cite or link to this item:
Title: Cationic polyelectrolyte amplified bead array for DNA detection with zeptomole sensitivity and single nucleotide polymorphism selectivity
Authors: Wang, C. 
Zhan, R.
Pu, K.-Y. 
Liu, B. 
Issue Date: 23-Aug-2010
Citation: Wang, C., Zhan, R., Pu, K.-Y., Liu, B. (2010-08-23). Cationic polyelectrolyte amplified bead array for DNA detection with zeptomole sensitivity and single nucleotide polymorphism selectivity. Advanced Functional Materials 20 (16) : 2597-2604. ScholarBank@NUS Repository.
Abstract: A highly sensitive strand specific DNA assay, which consists of a peptide nucleic acid (PNA) probe, a cationic conjugated polymer (PFVP), and selfassembled polystyrene beads in microwell arrays on silicon chip, is reported. PFVP, as an efficient signal amplifier and signal reporter, has been specially designed and synthesized to be compatible with commercial confocal microscopes for sensing on solid substrates. The assay operates on the net increase in negative charge at the PNA surface that occurs upon singlestranded DNA hybridization, which subsequently allows complex formation with the positively charged PFVP to favor energy transfer between the polymer and Cy5-labeled target. With maximized surface contact provided by bead arrays and signal amplification provided by PFVP, this assay allows detection of -300 copies of Cy5-labeled DNA using a commercial confocal microscope. In addition, the same strategy is also extended for label-free DNA detection with a detection sensitivity of 150 attomole. Excellent discrimination against single nucleotide polymorphism (SNP) is also demonstrated for both Cy5-labeled and label-free target detection. This study indicates that cationic conjugated polymers have great potential to be incorporated into the widely used microarray technology for simplified process with improved detection sensitivity. © 2010 WlLEY-VCH VerlagCmbH & Co. KCaA, Weinheirn.
Source Title: Advanced Functional Materials
ISSN: 1616301X
DOI: 10.1002/adfm.201000459
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 22, 2019


checked on Feb 5, 2019

Page view(s)

checked on Nov 17, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.