Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.10.029
Title: BMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice
Authors: Nie, H. 
Ho, M.-L.
Wang, C.-K.
Wang, C.-H. 
Fu, Y.-C.
Keywords: Bone regeneration
DNA delivery
Electrospinning
Hydroxyapatite
Nude mice
PLGA
Issue Date: Feb-2009
Source: Nie, H., Ho, M.-L., Wang, C.-K., Wang, C.-H., Fu, Y.-C. (2009-02). BMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice. Biomaterials 30 (5) : 892-901. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.10.029
Abstract: We studied three different types of scaffolds, encapsulating bone morphogenetic protein-2 (BMP-2) plasmid, in terms of their performances in bone regeneration in nude mice. The plasmid was loaded into fibrous matrices in three different ways: coating of naked DNA (Group A) or DNA/chitosan nanoparticles (Group B) onto scaffolds after fiber fabrication by dripping, and encapsulation of DNA/chitosan nanoparticles into scaffold by mixing them with PLGA/DCM solution before fiber fabrication (Group C). Their individual performances were examined by soft X-ray observation, histological analysis and immunostaining of bone tissue. In addition, the BMP-2 protein concentration and alkaline phosphatase (ALP) activity in serum were monitored. The results revealed that the bioactivity of BMP-2 plasmid released from all three kinds of scaffolds was well maintained; this eventually helped improve the healing of segmental defects in vivo. Interestingly, the three kinds of scaffolds released DNA or DNA nanoparticles in different modes and their performances in bone healing were diverse. These observations demonstrate that the in vivo performance of these newly developed DNA delivery devices correlates well with their in vitro release profiles. © 2008 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/63549
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2008.10.029
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

81
checked on Dec 14, 2017

WEB OF SCIENCETM
Citations

67
checked on Nov 18, 2017

Page view(s)

26
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.