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|Title:||Bacteria-surface interaction in the presence of proteins and surface attached poly(ethylene glycol) methacrylate chains|
|Citation:||Tedjo, C., Neoh, K.G., Kang, E.T., Fang, N., Chan, V. (2007-08). Bacteria-surface interaction in the presence of proteins and surface attached poly(ethylene glycol) methacrylate chains. Journal of Biomedical Materials Research - Part A 82 (2) : 479-491. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.a.31172|
|Abstract:||This study analyzes the adhesion behavior of the gram positive bacteria, Staphylococcus aureus (S. aureus), and the gram negative bacteria, Escherichia coli (E. coli), on polypyrrole (PPY) surfaces in the presence of poly(ethylene glycol) methacrylate (PEGMA) chains and plasma proteins (bovine serum albumin and bovine plasma fibrinogen) either preadsorbed on the film surface or in the bacterial suspension. Bacterial adhesion experiments performed in a suspension of bacterial cells and protein may give important insights on the behavior of bacterial adhesion in an in vivo environment. Protein adsorption and bacterial adhesion on PEGMA-grafted PPY films were reduced by about a factor of 2-4 compared with those on the pristine PPY films. In addition, the number of bacterial cells adhering on the substrate is dependent not only on the type of protein present, but also the sequence of exposure to the protein relative to the bacteria. Furthermore, bacteria-surface adhesion force was measured using the atomic force microscopy with increasing lateral force to detach the individual cell. The adhesion force of S. aureus is influenced by PEGMA and plasma protein modification and is significantly higher than that of E. coli for all substrates tested. The number of adherent cells on the substrate is shown to be directly correlated to the bacterial adhesion force. © 2007 Wiley Periodicals, Inc.|
|Source Title:||Journal of Biomedical Materials Research - Part A|
|Appears in Collections:||Staff Publications|
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