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Title: ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene
Authors: Wang, Y.
Liu, B. 
Issue Date: 2008
Citation: Wang, Y., Liu, B. (2008). ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene. Analyst 133 (11) : 1593-1598. ScholarBank@NUS Repository.
Abstract: A simple and sensitive method for ATP detection using a label-free DNA aptamer as the recognition element and ethidium bromide (EB) as the signal reporter is reported. The ATP-binding aptamer undergoes a conformational switch from the aptamer duplex to the aptamer/target complex upon target binding, which induces the fluorescence change of intercalated EB emission. Good selectivity between ATP and CTP, GTP or UTP has been demonstrated, which is due to the specific recognition between the ATP aptamer and ATP. Using EB alone as a signal reporter, the ATP detection limit was estimated to be ∼0.2 mM. When a light harvesting cationic tetrahedralfluorene was used as an energy donor to sensitize the intercalated EB emission, a 10-fold increase in detection limit and a 2-fold increase in detection selectivity was demonstrated. The sensitivity and selectivity of the tetrahedralfluorene sensitized assay is comparable to or better than most fluorescent ATP assays with multiple labels. © The Royal Society of Chemistry.
Source Title: Analyst
ISSN: 00032654
DOI: 10.1039/b806908e
Appears in Collections:Staff Publications

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