Please use this identifier to cite or link to this item:
|Title:||ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene|
|Citation:||Wang, Y., Liu, B. (2008). ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene. Analyst 133 (11) : 1593-1598. ScholarBank@NUS Repository. https://doi.org/10.1039/b806908e|
|Abstract:||A simple and sensitive method for ATP detection using a label-free DNA aptamer as the recognition element and ethidium bromide (EB) as the signal reporter is reported. The ATP-binding aptamer undergoes a conformational switch from the aptamer duplex to the aptamer/target complex upon target binding, which induces the fluorescence change of intercalated EB emission. Good selectivity between ATP and CTP, GTP or UTP has been demonstrated, which is due to the specific recognition between the ATP aptamer and ATP. Using EB alone as a signal reporter, the ATP detection limit was estimated to be ∼0.2 mM. When a light harvesting cationic tetrahedralfluorene was used as an energy donor to sensitize the intercalated EB emission, a 10-fold increase in detection limit and a 2-fold increase in detection selectivity was demonstrated. The sensitivity and selectivity of the tetrahedralfluorene sensitized assay is comparable to or better than most fluorescent ATP assays with multiple labels. © The Royal Society of Chemistry.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 15, 2018
WEB OF SCIENCETM
checked on May 8, 2018
checked on Apr 21, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.