Please use this identifier to cite or link to this item:
|Title:||Application of liquid-phase microextraction and gas chromatography-mass spectrometry for the determination of polychlorinated biphenyls in blood plasma|
|Authors:||Basheer, C. |
|Citation:||Basheer, C., Lee, H.K., Obbard, J.P. (2004-01-02). Application of liquid-phase microextraction and gas chromatography-mass spectrometry for the determination of polychlorinated biphenyls in blood plasma. Journal of Chromatography A 1022 (1-2) : 161-169. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2003.09.043|
|Abstract:||This study investigated the feasibility of applying liquid-phase microextraction combined with gas chromatography-mass spectrometry (GC-MS) to determine polychlorinated biphenyls (PCBs) in blood plasma. An efficient and simple extraction technique has been developed for the enrichment of PCBs from human blood plasma samples using single-step liquid-phase microextraction (LPME) in conjunction with a hollow fibre membrane (HFM). An eight PCB congener mixture was spiked into 2.5ml of blood plasma, and the solution was then adjusted to pH 10.5 with a salinity of 20% (w/v) prior to making the total volume to 5ml with ultrapure water. The porous HFM, filled with 3μl of organic solvent, was then immersed into the solution, which was continuously agitated at 700rpm for 30min. Extract (1μl) containing the pre-concentrated analytes was then injected into a GC-MS without further pre-treatment. Using an optimised extraction procedure, a large enrichment factor of the analytes, i.e. up to 241-fold was achieved in 30min. The procedure resulted in a relative standard deviation of <11% (n=6), and a linear calibration range from 2.5 to 150μg/l (r > 0.999), and detection limits between 0.07 and 0.94μg/l, respectively. To demonstrate the feasibility of the procedure, PCB concentrations were determined in actual blood samples collected from the local population in Singapore using the optimised LPME technique. © 2003 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Chromatography A|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 17, 2018
WEB OF SCIENCETM
checked on Oct 1, 2018
checked on Sep 29, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.