Please use this identifier to cite or link to this item: https://doi.org/10.1021/bm0700486
Title: Adsorption of plasmid DNA onto, N,N′-(dimethylamino)ethyl-methacrylate graft-polymerized poly-l-lactic acid film surface for promotion of in-situ gene delivery
Authors: Jiang, T.
Chang, J.B.
Wang, C.
Ding, Z.
Chen, J.
Zhang, J.
Kang, E.-T. 
Issue Date: Jun-2007
Source: Jiang, T., Chang, J.B., Wang, C., Ding, Z., Chen, J., Zhang, J., Kang, E.-T. (2007-06). Adsorption of plasmid DNA onto, N,N′-(dimethylamino)ethyl-methacrylate graft-polymerized poly-l-lactic acid film surface for promotion of in-situ gene delivery. Biomacromolecules 8 (6) : 1951-1957. ScholarBank@NUS Repository. https://doi.org/10.1021/bm0700486
Abstract: The surface of biodegradable poly-L-lactic acid (PLLA) film was modified with N,N'-(dimethylamino)ethyl-methacrylate (DMAEMA) via UV-induced graft copolymerization, and plasmid DNA molecules were adsorbed onto the surface of modified PLLA film by electrostatic interactions with cationic DMAEMA polymer. We characterized the structure of the modified PLLA film surface by Fourier transform infrared attenuated total reflection (FTIR-ATR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The weight-average molecular weight (Mw) of grafted DMAEMA polymer chains was estimated from the elution time of gel filtration chromatography. C.I. Acid Orange 7 dyeing results indicated that graft density of DMAEMA on PLLA film increased with the UV irradiation time and then reached a saturated value. DNA adsorption density was proportioned to graft density of DMAEMA. Mouse fibroblast L929 cell line was cultured on modified PLLA films, and cell viability and gene transfection efficiency were monitored after 2 days culture. It was found that the DMAEMA grafted PLLA film had obvious cytotoxicity to the cells. On the contrary, cytotoxicity of the surface was highly decreased after adsorption with plasmid DNA. This DNA adsorbed DMAEMA modified PLLA showed the ability to deliver DNA into mammalian cells cultured on the surface with high-transfection efficiency at a low DNA amount. The present results suggest that the DMAEMA grafted PLLA has potentiality to be used as a safe and effective gene delivery system in gene-activated materials. © 2007 American Chemical Society.
Source Title: Biomacromolecules
URI: http://scholarbank.nus.edu.sg/handle/10635/63445
ISSN: 15257797
DOI: 10.1021/bm0700486
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

25
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

22
checked on Nov 21, 2017

Page view(s)

19
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.