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Title: Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance
Authors: Lu, H.-F.
Chua, K.-N.
Zhang, P.-C.
Lim, W.-S.
Ramakrishna, S. 
Leong, K.W.
Mao, H.-Q.
Keywords: Cell-cell interaction
Functional maintenance
Galactose ligand
Hepatocyte spheroid
Issue Date: Jul-2005
Citation: Lu, H.-F., Chua, K.-N., Zhang, P.-C., Lim, W.-S., Ramakrishna, S., Leong, K.W., Mao, H.-Q. (2005-07). Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance. Acta Biomaterialia 1 (4) : 399-410. ScholarBank@NUS Repository.
Abstract: Functional maintenance of primary hepatocytes in culture can be improved by several distinct approaches involving optimization of the extracellular matrix microenvironment, media composition and cell-cell interactions, both homotypic and heterotypic. Using a galactose-decorated surface, we have developed a method to combine these two approaches by co-culturing rat primary hepatocyte spheroids with NIH/3T3 mouse fibroblast cells. Spheroids were performed by culturing hepatocytes for 3 days on galactosylated poly(vinylidene difluoride) membrane; NIH/3T3 cells were subsequently seeded and co-cultured with the spheroids. Results showed that although NIH/3T3 cells alone responded poorly to the galactosylated PVDF surface and displayed limited attachment, NIH/3T3 fibroblasts attached to the periphery of the hepatocyte spheroids and proliferated around them. Co-cultured hepatocyte spheroids exhibited significantly higher liver-specific functions as compared to spheroids cultured alone. Albumin secretion level in this co-culture system peaked on day 11, which was 1.8- and 2.9-times higher than the peak expression level in spheroid homo-culture control in serum-free (day 3) and serum-containing media (day 4), respectively. The albumin secretion function was maintained for at least two weeks; it was 5.1 (in serum-free medium) and 17.8 (in serum-containing medium) times higher than spheroid homo-culture on day 13. Similarly, the co-culture system also expressed approximately 5.5- and 3.1-times higher 3-methylcholanthrene-induced cytochrome P450 enzymatic activity on day 14 as compared to the homo-culture control in serum-free and serum-containing medium, respectively. In conclusion, this unique co-culture system demonstrated the synergistic roles of homotypic cell-cell interaction, heterotypic cell-cell interaction, cell-substrate interaction and soluble stimuli in hepatocyte functional maintenance. © 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Source Title: Acta Biomaterialia
ISSN: 17427061
DOI: 10.1016/j.actbio.2005.04.003
Appears in Collections:Staff Publications

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