Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10544-007-9071-5
Title: Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration
Authors: Chaw, K.C.
Manimaran, M.
Tay, F.E.H. 
Swaminathan, S. 
Keywords: Cell invasion
Cell migration
Extracellular matrices (ECM)
Matrigel coating
Microfluidic devices
Issue Date: Aug-2007
Citation: Chaw, K.C., Manimaran, M., Tay, F.E.H., Swaminathan, S. (2007-08). Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration. Biomedical Microdevices 9 (4) : 597-602. ScholarBank@NUS Repository. https://doi.org/10.1007/s10544-007-9071-5
Abstract: Three-dimensional (3-D) extracellular matrices (ECM) allow complex biochemical and biophysical interactions between cells and matrices. Unlike 2-D systems, 3-D models provide a better representation of the micro and local environments in living tissues for facilitating the physiological study of cell migration. Here, we report a microfluidic device based on polydimethylsiloxane (PDMS) for monitoring 3-D cell migration across ECM-coated microgaps with real-time light microscopy. We tracked the migration of the invasive MDA-MB-231 (mammary carcinoma) cells and mapped out their migration paths. It enabled us to quantify the percentage of migrated cells as well as migration information of individual cells. This wide spectrum of data acquisition is vital for elucidating the migration capabilities of different type of cells and to understand the basic mechanism involved in cancer metastasis. © Springer Science + Business Media, LLC 2007.
Source Title: Biomedical Microdevices
URI: http://scholarbank.nus.edu.sg/handle/10635/60688
ISSN: 13872176
DOI: 10.1007/s10544-007-9071-5
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