Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.b.31862
Title: Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering
Authors: Kai, D.
Prabhakaran, M.P. 
Jin, G.
Ramakrishna, S. 
Keywords: aligned nanofibers
anisotropy
cardiac tissue engineering
cell orientation
electrospinning
Issue Date: Aug-2011
Source: Kai, D., Prabhakaran, M.P., Jin, G., Ramakrishna, S. (2011-08). Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering. Journal of Biomedical Materials Research - Part B Applied Biomaterials 98 B (2) : 379-386. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.b.31862
Abstract: Cardiac tissue engineering (TE) is one of the most promising strategies to reconstruct the infarct myocardium and the major challenge involves producing a bioactive scaffold with anisotropic properties that assist in cell guidance to mimic the heart tissue. In this study, random and aligned poly(ε- caprolactone)/gelatin (PG) composite nanofibrous scaffolds were electrospun to structurally mimic the oriented extracellular matrix (ECM). Morphological, chemical and mechanical properties of the electrospun PG nanofibers were evaluated by scanning electron microscopy (SEM), water contact angle, attenuated total reflectance Fourier transform infrared spectroscopy and tensile measurements. Results indicated that PG nanofibrous scaffolds possessed smaller fiber diameters (239 ± 37 nm for random fibers and 269 ± 33 nm for aligned fibers), increased hydrophilicity, and lower stiffness compared to electrospun PCL nanofibers. The aligned PG nanofibers showed anisotropic wetting characteristics and mechanical properties, which closely match the requirements of native cardiac anisotropy. Rabbit cardiomyocytes were cultured on electrospun random and aligned nanofibers to assess the biocompatibility of scaffolds, together with its potential for cell guidance. The SEM and immunocytochemical analysis showed that the aligned PG scaffold greatly promoted cell attachment and alignment because of the biological components and ordered topography of the scaffolds. Moreover, we concluded that the aligned PG nanofibrous scaffolds could be more promising substrates suitable for the regeneration of infarct myocardium and other cardiac defects © 2011 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part B Applied Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/60423
ISSN: 15524973
DOI: 10.1002/jbm.b.31862
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

122
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

115
checked on Nov 14, 2017

Page view(s)

59
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.