Please use this identifier to cite or link to this item:
|Title:||Fibronectin immobilized by covalent conjugation or physical adsorption shows different bioactivity on aminated-PET|
|Citation:||Zhang, Y., Chai, C., Jiang, X.S., Teoh, S.H., Leong, K.W. (2007-03). Fibronectin immobilized by covalent conjugation or physical adsorption shows different bioactivity on aminated-PET. Materials Science and Engineering C 27 (2) : 213-219. ScholarBank@NUS Repository. https://doi.org/10.1016/j.msec.2006.03.013|
|Abstract:||To manipulate the cellular response to synthetic surfaces, extracellular matrix (ECM) proteins such as fibronectin (FN) and collagen are often immobilized on the surface to promote interaction between these ligands and the cell receptors. In this study we compared the biological properties of FN-decorated polyethylene terephthalate (PET) produced by two widely used immobilization techniques: adsorption and conjugation. As revealed by the micro-bicinchoninic acid (micro-BCA) assay and AFM, the modified surface topography was dependent on the immobilization methods. Adsorption method preserved the compact conformation of FN, reaching saturation when a monolayer of FN was formed. Covalent conjugation induced FN unfolding and fibrillogenesis, forming multiple layers of FN. Biological characterization by adhesion of baby hamster kidney 21 (BHK21) cells and enzyme-linked immunosorbent assay (ELISA) for active Arg-Gly-Asp (RGD) domains suggested that the difference in conformation of FN led to different bioactivities. Adsorption maintained a more active RGD domain, thereby promoting cell adhesion, whereas conjugation induced fibrillogenesis and blocked the access of RGD, consequently suppressing cell adhesion as the surface density of FN increased. This study suggests that in addition to choosing the nature of the adhesion molecule, the mode of immobilization may also significantly influence the bioactivity of the surface. © 2006 Elsevier B.V. All rights reserved.|
|Source Title:||Materials Science and Engineering C|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2018
WEB OF SCIENCETM
checked on May 28, 2018
checked on Jul 6, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.