Please use this identifier to cite or link to this item: https://doi.org/10.2166/ws.2011.109
Title: Rapid method for viable Cryptosporidium parvum oocysts detection
Authors: Chu, X.N. 
Hu, J.Y. 
Guo, H.L.
Tan, X.L.
Keywords: Cryptosporidium parvum
Detection
Filtration
FISH
Viability
Issue Date: 2011
Source: Chu, X.N., Hu, J.Y., Guo, H.L., Tan, X.L. (2011). Rapid method for viable Cryptosporidium parvum oocysts detection. Water Science and Technology: Water Supply 11 (6) : 737-744. ScholarBank@NUS Repository. https://doi.org/10.2166/ws.2011.109
Abstract: Cryptosporidium parvum is a waterborne coccidian protozoan parasite known to infect humans, resulting in an illness known as cryptosporidiosis. The widely used USEPA method 1622 to detect Cryptosporidium is time consuming, and unable to provide the information on oocysts viability and species. In order to develop a fast detection method for viable C. parvum oocysts, a 0.2 μm pore size hollow fiber membrane and 0.45 μm pore size disc membrane were used to filter tap water. Modified Immunomagnetic separation (IMS) purification was followed, and the IMS condition was optimized to shorten the purification time. Finally, fluorescence in situ hybridization (FISH) and FITC-conjugated monoclonal antibody (FMAb) staining were combined to detect viable oocysts, and results indicated that the viable oocysts detection by FISH/FMAb can be achieved in 20 min. The total viable oocysts detection time can be shortened to less than 1.5 h without affecting oocysts recovery. © IWA Publishing 2011.
Source Title: Water Science and Technology: Water Supply
URI: http://scholarbank.nus.edu.sg/handle/10635/59179
ISSN: 16069749
DOI: 10.2166/ws.2011.109
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