Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2012.02.060
Title: Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid
Authors: Ding, X.
Zhang, W. 
Cheng, D. 
He, J. 
Yang, K.-L. 
Keywords: Biosensor
Neonicotinoid
Peptide receptor
Phage display
SPR
Issue Date: 15-May-2012
Source: Ding, X., Zhang, W., Cheng, D., He, J., Yang, K.-L. (2012-05-15). Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid. Biosensors and Bioelectronics 35 (1) : 271-276. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2012.02.060
Abstract: By using phage display library, we identified two highly specific oligopeptide sequences RKRIRRMMPRPS and RNRHTHLRTRPR for binding neonicotinoids such as thiacloprid and imidacloprid. The former shows high affinity for thiacloprid whereas the latter shows high affinity for imidacloprid. Surprisingly, cross binding is minimal despite the similarity of the two molecules. To develop a neonicotinoid biosensor, these two oligopeptides are synthesized and immobilized on the surface of a surface plasmon resonance (SPR) chip with a bare-gold surface. This oligopeptide functionalized SPR biosensor can rapidly detect thiacloprid and imidacloprid in buffer solutions in a real-time manner. The limit of detection (LOD) for thiacloprid and imidacloprid is 1.2 μM and 0.9 μM, respectively. © 2012 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/59141
ISSN: 09565663
DOI: 10.1016/j.bios.2012.02.060
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