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|Title:||Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces|
|Authors:||Xu, F.J. |
|Citation:||Xu, F.J., Li, H.Z., Li, J., Teo, Y.H.E., Zhu, C.X., Kang, E.T., Neoh, K.G. (2008-12-01). Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces. Biosensors and Bioelectronics 24 (4) : 773-780. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.06.055|
|Abstract:||We report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizations (SI-ATRPs) from a resist-micropatterned Si(1 0 0) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1′-carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEG microdomains interact only and specifically with target anti-IgG, while the other PEG microregions effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored. © 2008 Elsevier B.V. All rights reserved.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
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