Please use this identifier to cite or link to this item: https://doi.org/10.1021/la800016h
Title: Colloidal crystals from surface-tension-assisted self-assembly: A novel matrix for single-molecule experiments
Authors: Wen, C.Y.
Kannan, B. 
Wohland, T. 
Ng, V. 
Issue Date: 4-Nov-2008
Citation: Wen, C.Y., Kannan, B., Wohland, T., Ng, V. (2008-11-04). Colloidal crystals from surface-tension-assisted self-assembly: A novel matrix for single-molecule experiments. Langmuir 24 (21) : 12142-12149. ScholarBank@NUS Repository. https://doi.org/10.1021/la800016h
Abstract: In this work, we develop a new method of creating colloidal crystals with cavities for the entrapment and long-term observation of single biomolecules. Colloidal crystals are first fabricated using surface-tension-assisted self-assembly. Surface tension helps to reduce the interparticle distance between dispensed colloids. Subsequently, the colloids are used as a matrix in which single fluorescently tagged molecules can be tracked using fluorescence microscopy. This method has a high efficiency of self-assembly for small volumes (4 μL) of colloidal suspensions (polystyrene colloids with diameters of 1000, 500, 200, and 100 nm) at low concentration (1% w/w). The spatial hindrance effect on the diffusion of molecules and their entrapment is discussed on the basis of fluorescence correlation spectroscopy results from the diffusion of molecules with different hydrodynamic radii in the cavities of colloidal crystals formed from micrometer- to nanometer-sized polystyrene spheres. Single horseradish peroxidase molecules turning over fluorescent products are tracked over a few seconds. This shows that colloidal crystals can be used to test the function of single molecules of enzymes and protein under controlled spatial confinement. © 2008 American Chemical Society.
Source Title: Langmuir
URI: http://scholarbank.nus.edu.sg/handle/10635/55322
ISSN: 07437463
DOI: 10.1021/la800016h
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

3
checked on Oct 8, 2018

WEB OF SCIENCETM
Citations

6
checked on Oct 1, 2018

Page view(s)

53
checked on Sep 29, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.