Please use this identifier to cite or link to this item:
|Title:||A fine match between the stereoselective ligands and membrane pore size for enhanced chiral separation|
|Authors:||Wang, H. |
Membrane pore size
|Citation:||Wang, H., Li, Y., Chung, T.-S. (2009-09). A fine match between the stereoselective ligands and membrane pore size for enhanced chiral separation. AIChE Journal 55 (9) : 2284-2291. ScholarBank@NUS Repository. https://doi.org/10.1002/aic.11858|
|Abstract:||A D,L-tryptophan separation factor of 12-15 and D-tryptophan yield of >95% have been successfully achieved through using human serum albumin (HSA) as the stereoselective ligand in an affinity ultrafiltration (UF) system. The obtained separation factor in this work is even higher than the intrinsic value of 8.5 of HSA. This synergism may arise from the fact that a fine match between the regular crystalline structure of HSA molecules and suitable pore size of membranes makes some HSA molecules be retained within the membrane cross-section, thus offering a second-stage binding opportunity for L-tryptophan molecules. Therefore, a simultaneous enhancement in separation factor and D-tryptophan yield has been fulfilled in this work. The feasibility of HSA regeneration after D,L-tryptophan separation has also been demonstrated through a series of pH adjustment experiments. This study reveals the applicability of HSA in affinity UF systems for chiral separation due to economization of material costs. © 2009 American Institute of Chemical Engineers.|
|Source Title:||AIChE Journal|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2018
WEB OF SCIENCETM
checked on Jun 26, 2018
checked on Jul 6, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.