Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/53805
Title: SER/THR PHOSPHORYLATION OF FLAGELLAR MOTILITY PROTEIN, FLIC AND ITS BIOLOGICAL EFFECTS IN PSEUDOMONAS AERUGINOSA PAO1
Authors: TANUJAA SURIYANARAYANAN
Keywords: p,s,f,
Issue Date: 24-Jan-2014
Source: TANUJAA SURIYANARAYANAN (2014-01-24). SER/THR PHOSPHORYLATION OF FLAGELLAR MOTILITY PROTEIN, FLIC AND ITS BIOLOGICAL EFFECTS IN PSEUDOMONAS AERUGINOSA PAO1. ScholarBank@NUS Repository.
Abstract: The focus of this study is on the biological effects of two potential serine/threonine phosphosites identified in the flagellar motility protein flagellin of Pseudomonas aeruginosa PAO1 .We report here that ser/thr mutation of FliC does not affect motility or chemotaxis but with significant affects on at three surface-associated phenomena -- biofilm formation, extracellular virulence factor secretion through Type 2 Secretion System (T2SS) and alginate secretion system. Flagellin phosphorylation affects the secretion system at a post-translational level. Flagellin phosphorylation also affected biofilm architecture, initial attachment and dispersal based on static and flow cell studies. Alginate secretion via a separate secretion system and related to the formation of biofilm matrix was also significantly affected. This study provides a link between ser/thr/tyr phosphorylation and virulence factor secretion and can improve our understanding of coordinated control of virulence mechanisms using Pseudomonas as a model bacterial pathogen.
URI: http://scholarbank.nus.edu.sg/handle/10635/53805
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